T4265

Sigma

 

Thrombin from bovine plasma

lyophilized powder, 175-350 NIH units/mg protein (biuret)

Synonym:Factor IIa
CAS Number:9002-04-4
Enzyme Commission (EC) Number:3.4.21.5   ( BRENDA | IUBMB )
EC Number:232-648-7
MDL number:MFCD00166053

Description

Analysis NoteActivity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
 The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Biochem/physiol ActionsSerine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Other NotesView more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Physical formLyophilized powder containing 75% sodium citrate, pH 5.8

Properties

formlyophilized powder
compositionProtein, ~25%
storage temp.−20°C

Safety

Safety Statements22-24/25
WGK Germany2
RTECSXO8950000

Related Products

SubstrateB1260, N-Benzoyl-Phe-Val-Arg 4-methoxy-β-naphthylamide hydrochloride
 S9009, Sar-Pro-Arg p-nitroanilide dihydrochloride
 B9385, Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride
 B4028, Boc-β-benzyl-Asp-Pro-Arg-7-amido-4-methylcoumarin hydrochloride
 B7632, N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride
InhibitorA9141, Antithrombin III from bovine plasma
 D0879, Diisopropylfluorophosphate
 A6191, Antipain dihydrochloride from microbial source
 A7388, Antithrombin III from human plasma
 P7626, Phenylmethanesulfonyl fluoride
 E7888, N-Acetyl-eglin C
 L2023, Leupeptin trifluoroacetate salt
 H7016, Hirudin from leeches
 H7380, Hirudin from leeches
 D7910, 3,4-Dichloroisocoumarin

References

reference, R. Biggs, ed. Human Blood Coagulation, Haemostasis and Thrombosis 2nd ed., Philadelphia , (1976), 722
MerckMerck 13,9461